Stability of canine factor VIII: coagulant activity in vitro.          下载免费PDF全文

P D Mansell  B W Parry 《Canadian journal of veterinary research》1989,53(3):264-267

A pilot study was undertaken to assess the stability of canine factor VIII:coagulant (FVIII:C) activity over three days, under various storage conditions (plasma at 4, 20 and 37 degrees C, whole blood at 4 and 20 degrees C). Blood collected from normal and hemophiliac dogs was used. Both plasma and whole blood samples appeared to be stable for up to 48 h at 4 and 20 degrees C. A subsequent study evaluated FVIII:C stability at 4 and 20 degrees C when stored as whole blood only. Samples were tested at 0, 24 and 48 h after collection. At 4 degree C there was a significant decline at 24 h (p less than 0.05), from 110% to 97% (mean values). Although the mean value was further decreased at 48 h (89%) this was not significant (p greater than 0.05). No significant change in FVIII:C activity was observed in whole blood stored at 20 degrees C for 24 or 48 h (110% and 107% respectively). These results suggest that canine whole blood samples collected into sodium citrate stored at 20 degrees C are adequate for routine FVIII:C assay for up to 48 h after collection.  相似文献   

Wastewater renovation and reuse: Frank M. D'Itri (editor). Volume 3, Pollution engineering and technology series. Marcel Dekker,Basel, Switzerland, 705 pp., SwFr. 130.00, ISBN 0-8247-6505-2     

Robert S. Mansell 《Agricultural Water Management》1977,1(4):361-363

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Association of fecal calprotectin concentrations with disease severity,response to treatment,and other biomarkers in dogs with chronic inflammatory enteropathies          下载免费PDF全文

Romy M. Heilmann  Nora Berghoff  Joanne Mansell  Niels Grützner  Nolie K. Parnell  Corinne Gurtner  Jan S. Suchodolski  Jörg M. Steiner 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(2):679-692

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Effects of the gill monogenean Zeuxapta seriolae (Meserve, 1938) and treatment with hydrogen peroxide on pathophysiology of kingfish, Seriola lalandi Valenciennes, 1833     

Mansell B  Powell MD  Ernst I  Nowak BF 《Journal of fish diseases》2005,28(5):253-262

Infections by the gill fluke Zeuxapta seriolae are a serious concern for sea cage aquaculture of kingfish, Seriola lalandi. The present study aimed to determine the pathophysiological effects of a progressive infection with Z. seriolae and the effects of treatment with hydrogen peroxide. For the progression of infection study, infected fish were taken from a sea cage farm, treated to remove parasites and then infected by cohabitation with heavily infected fish. Samples were taken at 2-week intervals for 8 weeks. Infection intensity peaked at 4 weeks post-infection (mean intensity 565.9) and the number of mature worms (2 mm fixed length or larger) peaked at 6 weeks post-infection. Attachment of Z. seriolae appeared to cause little localized pathology; however, the occurrence of hyperplastic lamellae increased as the infection progressed. Haemoglobin concentrations were negatively correlated with Z. seriolae intensity and were lower than controls at 4 weeks (35.8% decrease) and 6 weeks (57.4% decrease) post-infection. Blood lactate concentration and plasma osmolality increased throughout the course of infection. For the effect of treatment experiment, groups of infected and non-infected fish were sampled either before or after treatment with hydrogen peroxide. Treated fish from both infected and uninfected groups had increased plasma lactate, osmolality and pH compared with pre-treatment groups. Treatment with hydrogen peroxide appeared to have acute effects on fish health but the magnitude (e.g. lactate, osmolality) and extent of the effects (e.g. haemoglobin) was much less than that caused by chronic infection with Z. seriolae.  相似文献   

Hydrogen-Bond Breaking and Proton Exchange in Collisions of Gaseous Formic Acid with Liquid Sulfuric Acid     

JK Klassen  GM Nathanson 《Science (New York, N.Y.)》1996,273(5273):333-335

Gas-liquid scattering experiments provide direct observations of the fate of hydrogen-bonding molecules striking the surfaces of acidic liquids. Collisions of gaseous formic acid with concentrated sulfuric acid show that impinging monomers (HCOOH and DCOOD) scatter inelastically from the interface or become trapped by surface H2SO4. Most trapped DCOOD molecules undergo proton exchange before desorbing from the acid, indicating that gas-surface accommodation almost always leads to reaction with H2SO4 molecules. This proton transfer is not inhibited by dimerization of the formic acid: The dimers readily undergo intramolecular hydrogen bond cleavage and D-H exchange before desorbing from the acid.  相似文献   

Factors affecting colostrum quality in Australian pasture‐based dairy herds          下载免费PDF全文

GM Chuck  PD Mansell  MA Stevenson  MM Izzo 《Australian veterinary journal》2017,95(11):421-426

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Identification of lactoferrin and glutamate receptor‐interacting protein 1 in bovine cervical mucus: A putative marker for oestrous detection          下载免费PDF全文

WY Lee  MH Park  KW Kim  H Song  KB Kim  CS Lee  NK Kim  JK Park  BC Yang  KB Oh  GS Im  HJ Chung 《Reproduction in domestic animals》2017,52(1):16-23

Accurate detection of oestrus is important for artificial insemination. The aim of this study was to identify oestrous‐specific bovine cervical mucus proteins that could be used to determine the optimal time for artificial insemination. Non‐oestrous and controlled internal drug release (CIDR)‐induced oestrous‐stage mucus proteins were purified and subjected to surface‐enhanced laser desorption/ionization time‐of‐flight mass spectrometry, sodium dodecyl sulphate polyacrylamide gel electrophoresis and MALDI‐TOF/TOF. Among differentially expressed proteins, lactoferrin (LF) and glutamate receptor‐interacting protein 1 (GRIP1) showed a twofold increase during the CIDR‐induced oestrous stage compared to the levels in non‐oestrous stage in bovine cervical mucus. The RT‐PCR, Western blotting and immunohistochemistry results showed that LF and GRIP1 expression was significantly increased during the oestrous stage in the uterus. This study demonstrated that bovine LF and GRIP1 exist during the oestrous stage, but not during the non‐oestrous stage, suggesting that cervical mucus LF and GRIP1 are useful oestrous detection markers in cattle.  相似文献   

Characterization of the cutaneous mycobiota in healthy and allergic cats using next generation sequencing          下载免费PDF全文

Courtney Meason‐Smith  Alison Diesel  Adam P. Patterson  Caitlin E. Older  Timothy J. Johnson  Joanne M. Mansell  Jan S. Suchodolski  Aline Rodrigues Hoffmann 《Veterinary dermatology》2017,28(1):71-e17

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